High-throughput method
WebApr 14, 2024 · Accurately and rapidly counting the number of maize tassels is critical for maize breeding, management, and monitoring the growth stage of maize plants. With the … WebHigh-throughput sequencing, also known as next-generation sequencing (NGS), is the comprehensive term used to describe technologies that sequence DNA and RNA in a …
High-throughput method
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WebSep 24, 2024 · We have developed a simple 96-well plate-based high-throughput DNA extraction method that is applicable to many plant species. The method involves a simple incubation of plant tissue samples in a DNA extraction buffer followed by a neutralization step. With the addition of a modified PCR buffer, the extracted DNA enabled the robust ... WebJul 31, 2024 · In conclusion, our platform provides a high-throughput method to interrogate a wide variety of protein-DNA interactions. The utilities of our DocMF platform can be extended to other applications, such as on-chip identification of off-target sites for a CRISPR-Cas system, single-stranded DNA cleavage sites, or TF binding motifs.
WebSep 11, 2015 · The Gauss-peak spectra (GPS) method represents individual pigment spectra as weighted sums of Gaussian functions, and uses these to model absorbance spectra of phytoplankton pigment mixtures. We here present several improvements for this type of methodology, including adaptation to plate reader technology and efficient model fitting … WebDirect infusion of lipid extracts into the ion source of a mass spectrometer is a well-established method for lipid analysis. In most cases, nanofluidic devices are used for sample introduction. ... based on sample infusion from a chromatographic pump can offer a s … Robust and high-throughput lipidomic quantitation of human blood samples ...
WebNov 10, 2024 · Compared with traditional strain screening and plate confrontation experiments, the one-step high-throughput method used to screen endophytic bacteria displaying antagonistic activity against pathogenic fungi in rice seeds can complete all the required processes in 4–5 days. High-throughput screening (HTS) is a method for scientific experimentation especially used in drug discovery and relevant to the fields of biology, materials science and chemistry. Using robotics, data processing/control software, liquid handling devices, and sensitive detectors, high-throughput … See more The key labware or testing vessel of HTS is the microtiter plate, which is a small container, usually disposable and made of plastic, that features a grid of small, open divots called wells. In general, microplates for HTS have either … See more Automation is an essential element in HTS's usefulness. Typically, an integrated robot system consisting of one or more robots transports assay-microplates from station to station for sample and reagent addition, mixing, incubation, and finally readout or … See more Unique distributions of compounds across one or many plates can be employed either to increase the number of assays per plate or to reduce the variance of assay results, or … See more • Chemoproteomics • Compound management • DNA-encoded chemical library See more To prepare for an assay, the researcher fills each well of the plate with some biological entity that they wish to conduct the experiment upon, such as a protein, cells, … See more With the ability of rapid screening of diverse compounds (such as small molecules or siRNAs) to identify active compounds, HTS … See more HTS is a relatively recent innovation, made feasible largely through modern advances in robotics and high-speed computer technology. It still takes a highly specialized and expensive screening lab to run an HTS operation, so in many cases a small- to moderate … See more
WebJul 13, 2024 · We developed a high-throughput hybridisation-based targeted enrichment protocol for third-generation sequencing application. Long fragments of >7 kb can be …
WebOct 17, 2024 · For high-throughput analyses, methods are preferred for short-time characterizations in which the spheres can be used without further preparation. This … grand oaks drive crestwood kyWebJan 1, 2004 · The DNA microarray technology, the most commonly used method for high-throughput gene expression profiling, has been touted as an integrative tool that provides insights into regulatory pathways. However, the physiology community has been slow in acceptance of these techniques because of early failure in generating useful data and the … grand oaks crystal lake park districtWebApr 27, 2024 · In addition, high-throughput techniques can be used in bacteriophage-based experiments because bacteria grow more rapidly than mammalian cells, thus the evaluation process is faster and more effective. Further improvements in experimental efficiency will accelerate the development of novel antiviral materials. chinese imari porcelain strainer bowlWebSep 23, 2010 · The rapid SA extraction method by direct boiling of leaf discs further reduced the cost and time required for the biosensor Acinetobacter sp. ADPWH_lux-based SA estimation, and allowed the screening for npr1 suppressors that accumulated less SA than npr1 after pathogen infection in a high-throughput manner. grand oaks early learning centre lilydaleWebJun 5, 2024 · To address these shortcomings, we developed Visual Cell Sorting, a flexible and simple high-throughput method that uses commercial hardware to enable the investigation of cells according to visual phenotype. Visual Cell Sorting is an automated platform that directs a digital micromirror device to mark single live cells that express a … grand oaks culinaryWebJul 13, 2024 · We developed a high-throughput hybridisation-based targeted enrichment protocol for third-generation sequencing application. Long fragments of >7 kb can be efficiently enriched in 16× sample multiplexing resulting in >6 kb HiFi reads. Issue Section: Large scale sequencing methods INTRODUCTION grand oaks early learning centreWebNov 6, 2024 · Here we describe a strategy for ultra high-throughput joint analysis of transcriptome and accessible chromatin that could be used to study millions of individual … chinese imax theatre