Jeg 3 cell line

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August undefined, 2024

WebFigure 10 The effect of overexpression or silencing of hsa-miR-29b-3p, hsa-miR-519c-3p and hsa-miR-520a-5p on cell proliferation in JEG-3 and JAR cells. Viability of JEG-3 and JAR cells transfected with hub DEM mimics or inhibitors measured by CCK-8 (A–D) and EdU assays (E and F). The percentage of Edu positive JEG-3 and JAR. Web29 nov 2024 · The BeWo cell line is used to study placental function, since it forms syncytium and secretes hormones after treatment with cAMP or forskolin. In contrast, the …

JEG-3 Trophoblast Cells Producing Human Chorionic …

WebCellosaurus JEG-3 (CVCL_0363) Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE). Part of: COSMIC cell lines project. Characteristics: Established by serial cloning into hamster cheek pouches. Doubling time: ~24 hours (DSMZ=ACC-463); ~36 hours (CLS). WebOn day 0, JEG-3 cultures received resting or stimulated PBMCs in a 1:25 or 1:50 ratio of JEG-3 cells to PBMCs. This ratio was calculated based on the initial number of JEG-3 seeded in the culture plates. A low dose of IL-2 (Sigma) was added at this time and again on day 3 to promote PBMC survival. Treated JEG-3 cell co-cultures were performed ... rediff ukraine

JEG-3 - German Collection of Microorganisms and Cell Cultures …

WebJEG-3 (CLS catalog number 300222) Biosafety level: 1: Expression / Mutation / Susceptibility ... Successful preservation of cells is a top priority given that losing a cell … Web1 dic 2016 · Results: Our results showed that HTR-8/SVneo cell line contains two populations of cells suggesting the presence of trophoblast and stromal/mesenchymal cells. While all cells in BeWo, JEG-3 and ... Web13 apr 2024 · To verify the function of miR-526b-5p on the trophoblast cells and explore the intrinsic molecular mechanism, transcriptome sequencing was performed on the HTR8 cells overexpressing miR-526b-5p. The miR-526b-5p overexpressing HTR8 cell line was used as the experimental group. The pCD513B-1 empty vector cell line was transfected as the … rediff top chef

Effects of selective serotonin-reuptake inhibitors (SSRIs) in JEG-3 …

Inhibition of DLX4 promotes apoptosis in choriocarcinoma cell lines.

Web10 nov 2006 · The JEG-3 cell line are human choriocarcinoma cells derived from malignant placental tissues. As in placenta, JEG-3 cells endogenously express aromatase activity at detectable levels (Bahn et al., 1981) and the cyp19 gene expression is under the control of a distal promoter I.1 located adjacently upstream of exon I.1 in the gene (Means et al ... WebNational Center for Biotechnology Information rice fielderWeb16 giu 2015 · The JEG-3 choriocarcinoma cell line was obtained from the Cell Bank of the Chinese Academy of Sciences. The cells were routinely maintained in Dulbecco’s Modified Eagle Media: Nutrient Mixture F-12 (DMEM/F12) culture media supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin and incubated in 5% CO 2 , 95% air at 37 °C. rice field farming

"Web10 nov 2006 · The JEG-3 cell line are human choriocarcinoma cells derived from malignant placental tissues. As in placenta, JEG-3 cells endogenously express aromatase activity … " - Jeg 3 cell line

Jeg 3 cell line

Toxins Free Full-Text α-Solanine Inhibits Proliferation, Invasion ...

WebTo give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal … Web31 mar 2024 · The choriocarcinoma-derived JEG-3 cell-line (ATCC HTB-36, Manassas, VA, USA), was grown as recommended by ATCC: ... JEG-3 cells in 2.5% FBS released similar levels of hCG, hPL, and estradiol to JEG-3 cells in 10% FBS, and thus maintain the endocrine function of human placenta.

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Web12 nov 2024 · The JEG-3 cell line was transfected with pSpCas9 vector cloned with each sgRNA and analysed as follows. First, HLA-G expression was measured by WB and FC on JEG-3 edited cells. Web"1A6 Cell;1A6复苏细胞系(STR基因鉴定图谱) BS-C02767937S 背景信息：详见相关文献介绍产品包装：复苏：T25培养瓶(1瓶)或冻存：1ml冻存管(2支) 生长特性：贴壁或悬浮，详见说明书部分 1A6 Cell;1A6复苏细胞系(STR基因鉴定图谱) 很多

Web1 gen 2024 · JEG-3 is another independent human choriocarcinoma cell line reported first in 1971 [9]. It is mostly considered a model for invasive trophoblast cells [ 6 ]. JEG-3 … Web15 mar 2024 · After electroporation and drug selection, a successful HLA-G knockout JEG-3 cell line (JEG-3 KO cells) was established, and its efficacy was first validated by PCR and western blotting . Additional functional validation was performed on the well-established functions of HLA-G, including the inhibition of natural killer cell cytotoxicity and the …

http://hs.read.cnki.net.dr2am.wust.edu.cn/web/Journal/Article/WJDE202411006.html?__dp=https WebCell line: JEG-3 : DSMZ no.: ACC 463: Species validation: human (Homo sapiens)Cell type: choriocarcinoma : Origin: established from a human gestational choriocarcinoma …

Web13 mar 2024 · The culture of JEG-3 cells (a human choriocarcinoma cell line) has been described previously . To study the cytotoxic effect of α-solanine on JEG-3 cells, cells were cultured in 96-well plates overnight, followed by treatment with different concentrations of α-solanine (10, 20, 30, 40 and 50 μM) or vehicle (0.5% v/v DMSO) for 24 h.

Web19 lug 2024 · Here, we present a comparative study on the functional aspects of the choriocarcinoma cell lines BeWo, JAR and Jeg-3, as well as the first trimester … rediff ufcWeb1 dic 2024 · In contrast, the JEG-3 cell line fails to undergo substantial fusion. Therefore, BeWo and JEG-3 cells were used to identify a set of genes responsible for trophoblast … rediff twitch freeWebIn the present study, we investigated DLX4 mRNA and protein expression in both normal placental tissues and human choriocarcinoma cell lines. Also, using RNA interference (RNAi) technique, we knocked down the expression of DLX4 and examined apoptosis in JEG-3 cells. Our studies demonstrated that DLX4 RNAi inhibited DLX4... ricefield flatsedgeWebHuman chorionic gonadotrop significantly improved chemotherapy efficacy for the treatment of malignant trophoblastic tumors- Read the full article online rediffusion 14Web9 mag 2024 · The miR-371-3 cluster (including miR-371, -372, and -373) is expressed in primary trophoblast cells, JEG-3 cells, and their hybrids, but not in immortalized HTR-8/SVneo Mice have homologous ... regulation of Wnt/ β-catenin signaling pathway by miR-424-5p and affects invasion and migration of the trophoblastic cell line HTR-8/SVneo rediff twitch pas de sonWeb1 mag 2002 · Due to the metabolic property demonstrated in this study, the JEG-3 cell line belongs to a little group of transformed and established cell lines experimentally shown … rediffuaion echappes belleWeb4 mar 2016 · Here, we show that the trophoblast JEG-3 cell line recapitulates the morphological and secretory phenotypes associated with primary syncytiotrophoblasts when cocultured in 3D with microvascular endothelial cells. In addition, we show that this coculture system recapitulates the in vivo ability of syncytiotrophoblasts to resist … rediffusion 23