Two ambion silencer select sirna

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August undefined, 2024

WebDescription. The Silencer™ Select siRNAs are classic 21-mers which incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemistry. A … WebSilencerR Select siRNA targeting the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene (Ambion positive control) as an example in this chapter. SilencerR Select siRNAs are designed

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WebJan 11, 2024 · The long non-coding RNA (lncRNA) NKILA, localized to 20q13.31, is a negative regulator of NF-κB signaling implicated in carcinogenesis. As a CpG island is embedded in the promoter region of NKILA, it is hypothesized as a tumor suppressor lncRNA silenced by promoter DNA methylation in non-Hodgkin’s lymphoma (NHL). … WebSilencer Select 设计算法. 包含>90 种不同的序列和热力学参数. 与上一代 siRNA 设计算法相比，预测准确性提高 28%. 给出的 siRNAs 沉默效率比其他供应商的修饰和未修饰 siRNAs … ordinary unsecured creditors

Silencer Select Pre-designed siRNA Silencer Select Validated …

WebThe three testing siRNA are of Ambion Silencer Select, and have been previously validated either by the supplier or by other lab members. RT-qPCR was used to confirm downregulation of the target gene. WebAmbion™ AM16708 Silencer™ Select siRNAs are the result of recent improvements in siRNA design combined with novel chemical modifications yielding unrivaled knockdown … WebSilencer® Select Negative Control #2 siRNA Store at or below –20°C. Catalog #: 4390847 Amount: 40 nmol Appearance: Powder ... Ambion siRNA products are manufactured … ordinary utopia

Gene silencing. I would like to know if anyone has used before the ...

Silencer® Select siRNA Thermo Fisher Scientific - JP

WebCav-2 was discovered as a 20 kDa protein co-purified with adipocyte-derived caveolar membranes. 7 Cav-2-encoded gene is located on human chromosome 7q31.1. 7 Cav-2 can form a stable hetero-oligomeric complex of 14–16 molecules into a large macromolecular complex, which defines caveolar architecture at the plasma membrane. 6,7 In the … WebLife Technologies guarantees that when you purchase two Silencer Select Pre-designed siRNA to the same target, then those two siRNAs will silence the target mRNA by 70% or … how to turn off lights on razer cynosa chromaWeb27mer Dicer-substrate duplex : higher potency & minimal interferon response. Guaranteed gene knockdown (≥70%) Each of our siRNA products come as a kit. It contains 3 unique 27mer siRNA duplexes (2 nmol each), one negative control and one buffer. Each gene specific siRNA is labeled as the kit catalog number plus a/b/c. ordinary utility patent

"WebMar 14, 2024 · Description Silencer™ siRNA Libraries feature combinations of Silencer Pre-designed siRNAs, and when available, Silencer Validated siRNAs, in 96-well or 384-well plates. The design of each siRNA has been optimized for potent and specific silencing. The siRNA selection process yields siRNAs that are effective at low concentrations, enabling … " - Two ambion silencer select sirna

Two ambion silencer select sirna

Ambion™ Silencer™ Pre-Designed siRNA 5 nmol Ambion™ …

WebThe following siRNAs were employed: siRNA targeting REDD1 (#s29166; Ambion, Austin, TX, USA), non-targeting siRNA (#4390843; Invitrogen), and positive control siRNA against GAPDH (#4390849; Invitrogen). Gene knockdown efficiency was evaluated by quantitative reverse transcription-PCR on ABI Fast 7500 System (Applied Biosystems). WebThe Silencer Select Human Membrane Trafficking siRNA Library contains 426 Ambion™ Silencer Select siRNAs targeting 142 genes. This library of highly potent, chemically …

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WebRecommendations. Documents. FAQ. Ambion™ Silencer ™ SelectNegative Control #2 siRNA is extensively validated and an ideal control for many aspects of an siRNA … WebSilencer™ Select Pre-Designed siRNAs are guaranteed-to-silence based on their proven design while the Silencer™ Select Validated siRNAs are functionally tested to reduce …

WebDescription Silencer™ siRNA Libraries feature combinations of Silencer Pre-designed siRNAs, and when available, Silencer Validated siRNAs, in 96-well or 384-well plates. The … Web4 AY036118 Silencer Select Pre-designed, Validated, and Custom siRNA in Standard, HPLC, and In-vivo Ready Purities. 登录. 没有账户？建立账号注册. 产品. 抗体; Custom DNA …

Web新デザインアルゴリズムにより、有効siRNAの予想精度が25%超改善します。. 第一世代および第二世代のsiRNAデザインと比較して、その能力は最高100倍。. siRNAによるフェ …

Web1. Briefly centrifuge the tube or plate to ensure that the dried siRNA is at the bottom of the tube. 2. Resuspend the 5 nmol siRNA using 50 μL of the nuclease-free water provided for …

WebHow in perform siRNA transfection with Lipofectamine RNAiMAX protocol. Superior siRNA/miRNA childbirth or gene knockdown. ordinaryus profesörWebThe 96-well Silencer Select Human Druggable Genome siRNA Library V4 and Silencer Select Human ... Ambion † A part of Life Technologies Corporation † +1 800-888-8804 † www.ambion.com † e-mail: [email protected] Cat # (P/N) 4397920 Page 2 of 4 Lot #AMO02279 07/13/2010 Resuspension of siRNAs Centrifuge each plate at low speed ... how to turn off light on iphone 12WebAmbion, Inc., a part of Life Technologies Corporation • +1 800-888-8804 • www.ambion.com • e-mail: [email protected] Catalog #: Various 2/5/2010 Catalog #: Various Silencer® … how to turn off light on iphone 11WebSeeds from infiltrated plants were selected on standard MS medium (Murashige and Skoog 1962) ... (MegaScript; Ambion) ... Then, 2 μL of siRNA PDS (1 μM) and 0.3 μL of RNasin were added to the reaction, and incubated for 30 min at room temperature before addition of 3.2 μL of substrate cocktail (1 μL of 20 mM ATP, 1 μL of 32 P ... how to turn off lights on razer huntsman teWebA30148. Silencer ™ Select Human Long Non-coding RNA (lncRNA) siRNA Library. 1 set (96-well) A30149. Silencer ™ Select Human Long Non-coding RNA (lncRNA) siRNA Library … how to turn off lights on cyberpower mouseWebAug 7, 2024 · Four biological replicates per siRNA in two independent experiments (two biological replicates per ... human HEK293 cells were reverse transfected with siRNAs from the Ambion Silencer Select siRNA library, and after 48 h, a second transfection was performed with the minigenome assay components including a control-expression … how to turn off lights on cyberpower keyboardWebThe Silencer Select siRNA design algorithm was used to design 155 siRNAs to 40 different targets. These siRNAs were tested side by side with siRNAs designed using the previous … ordinary usually 違い